Fig. 3: Simultaneous screening of CTCF binding sites with a truncated guide.
From: Multi-locus CRISPRi targeting with a single truncated guide RNA
a Guide selection strategy based on top Homer generated motif from CTCF ChIP in Jurkat cells. b CTCF targeting schematic of a single 10nt guide directing CRISPRi to many motifs simultaneously (CTCF-bound and unbound). c Target site distribution (black) and CTCF occupancy based on Jurkat ChIP (blue) for each of the 10nt guide sequences. d, e Guide enrichment and depletion in pooled screen for the indicated cell lines. Pooled screens were run in triplicate, while K562 was in duplicate. Data are mean +/− SD. CTCF promoter targeting full length guide knockdown (KD) used as a control. Source data are provided as a Source Data file.
