Fig. 6: Lymphatic drainage immunomodulates the hematoma niche.
From: Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs
a Representative Wright-Giemsa stain of murine tibias on day 2 post-surgery. The right 3 images are higher magnifications of the regions boxed in the corresponding image left. Black and red arrows respectively indicate neutrophils and macrophages. Scale bars, 50 um (left), 500 um (right). b Quantitative analysis of neutrophils and macrophages in endosteum, periosteum, and bone marrow (n = 5/group). c FACS gating strategy for myeloid cells (CD45 + CD11b + ), monocytes (CD45 + CD11b + Ly6G − F4/80 − ), neutrophils (CD45 + CD11b + Ly6G + F4/80 − ), macrophages (CD45 + CD11b + Ly6G − F4/80 + ), Ly6Chigh monocytes (CD45 + CD11b + Ly6G − F4/80− Ly6Chigh), M1-type macrophages (CD45 + CD11b + Ly6G − F4/80 + CD86 + ) and M2-type macrophages (CD45 + CD11b + Ly6G − F4/80 + CD206 + ). d Quantitative analysis of monocyte and Ly6Chigh monocyte in hematoma niche by flow cytometry. e Quantitative analysis of neutrophils in hematoma niche by flow cytometry. f Quantitative analysis of macrophages and the ratio of M2-type to M1-type of macrophages in hematoma niche by flow cytometry. g The concentration of pro-inflammatory cytokines in hematoma homogenate on days 1, 2, and 3 post-surgery by ELISA. h The concentration of growth factors in hematoma homogenate on days 1, 2, and 3 post-surgery by ELISA. Data are means ± SD. In d-f, n = 4 in the sham group and n = 5 in the VEH and CLO groups, one-way ANOVA was used for sham, F-1d, and CLO-1d groups, whereas two-sided Student’s t-test was utilized for F−2d versus CLO-2d and F-3d versus CLO-3d. In g, h, n = 3 wells with biological replicates in the control, sham, VEH, and CLO groups, one-way ANOVA. Source data are provided as a Source Data file.
