Fig. 7: Draining lymphatic fluid inhibits OBs and BMSCs.

a Surgical procedures of thoracic lymph duct cannulation. The thoracic duct was retracted at first and then cannulated by a polyethylene tubing primed with heparinized saline. Lymph was collected by anticoagulant tube. b Scheme of an experimental procedure in vitro for OBs and BMSCs treated with 20% lymph CM. The thoracic duct lymph from sham, VEH, and CLO groups were collected to generate lymph CM. Rat BMSCs and OBs were cultured with 20% lymph CM for 24 h and subjected to Elisa, growth and proliferation analyses. The scheme was modified from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 4.0 Generic License (https://creativecommons.org/licenses/by/4.0/). c The concentration of pro-inflammatory cytokines in 20% lymph CM on days 1 and 2 post-surgery by ELISA. d The concentration of growth factors in 20% lymph on days 1 and 2 post-surgery by ELISA. e Rat OBs were cultured with 20% lymph CM for 24 h and the growth of rat OB was observed under a microscope. Scale bars, 50 um. f The cell count of rat OBs cultured with 20% lymph CM for 24 h. g Rat OBs were cultured with 20% lymph CM for 24 h and the cell proliferation was evaluated using a CCK8 kit. h Rat BMSCs were cultured with 20% lymph CM for 24 h and the growth of rat BMSCs was observed under a microscope. Scale bars, 50 um. i The cell count of rat BMSCs cultured with 20% lymph CM for 24 h. j Rat BMSCs were cultured with 20% lymph CM for 24 h and the cell proliferation was evaluated using a CCK8 kit. Data are means ± SD. In c-d, f-g, i-j, n = 3 wells with biological replicates in the control, sham, VEH and CLO groups, one-way ANOVA. Source data are provided as a Source Data file.