Fig. 9: Lymphatic platelet thrombolysis benefits senile fracture healing.
From: Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs
a Scheme of experimental procedure for senile fracture with clopidogrel intervention. The scheme was modified from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 4.0 Generic License (https://creativecommons.org/licenses/by/4.0/). b Immunofluorescence staining and quantitative analysis of LPT (n = 8/group). The right images are higher magnifications of the regions boxed in white in the corresponding image left. Scale bars, 200 mm (left), 50 mm (right). c Representative NIR-ICG images at hindlimb after ICG injection at day 7 post-fracture and quantitative analysis of draining lymphatic clearance (n = 8/group). d, e Representative NIR-ICG images showing ICG signals in a draining LV at day 7 post-fracture. f The number of LVs with or without a lymphatic pulse at day 7 (n = 8/group, two-sided and adjusted Pearson’ chi-squared test with P < 0.001, multiple comparisons with α’=0.0125). g Ultrasound images and quantification of PLNs at day 7 post fracture (n = 8/group). Scale bars, 1 mm (top and bottom). h The gross anatomy of isolated PLNs. Scale bars, 1 cm. i Representative photographs of hindlimb at day 2 post-fracture. Scale bars, 1 cm. j The relative swelling ratio of mouse hindlimb in 7 days after fracture (n = 8/group at each time point, two-sided analysis of variance in repeated measurement design followed the simple effect test). k Analysis of PWTL at day 1 (n = 8/group). l Representative Micro-CT images and quantificative analysis of callus at day 14 post-fracture (n = 8/group). m Representative histomorphological images of ABH-stained sections at day 14 post-fracture. The red, orange and black asterisks respectively indicate fiber, cartilage, and woven bone. The bottom images are higher magnifications of the regions boxed in black in the corresponding image above (n = 6 in the VEH-O group and n = 7 in the CLO-O group). Scale bars, 200 um (top), 100 um (bottom). n Callus composition in (m) were analyzed. o Analysis of biomechanical testing (n = 7 in the VEH-O, and n = 8 in the CLO-O group). Data are means ± SD. In b, c, g and k, one-way ANOVA. In l, n and o, two-sided Student’s t-test. Source data are provided as a Source Data file.
