Fig. 1: KIM-1 is markedly upregulated in UUO model, folic acid model and adenine model mice. | Nature Communications

Fig. 1: KIM-1 is markedly upregulated in UUO model, folic acid model and adenine model mice.

From: High-density lipoprotein nanoparticles spontaneously target to damaged renal tubules and alleviate renal fibrosis by remodeling the fibrotic niches

Fig. 1

a Representative immunofluorescence images(left) and the Pearson correlation coefficient(right) of colocalization of KIM-1(red) and LTL (green) in the kidney of control, UUO model, folic acid model and adenine model mice. Scale bars, 100 µm. Data are mean ± SD (n = 3 independent samples). b Western blot analysis of KIM-1 protein expression in the kidney of control, UUO model, folic acid model and adenine model mice. The value of 60 and 55 kDa are referred to the predicted protein weight of KIM-1 and α-tubulin. α-tubulin was used as internal control. n = 3 biologically independent samples. c Semi-quantification of KIM-1 protein expression by Image J based the western blots shown in b and it was normalized with corresponding α-tubulin signal. Data are mean ± SD (n = 3 independent samples, two-tailed unpaired t-test). d Real-time qPCR analysis of Havcr1 mRNA expression level in kidney of control, UUO model, folic acid model and adenine model mice. Data are displayed as normalized fold expressions relative to control group, and Acta mRNA was used as internal control. Data are mean ± SD (n = 4 independent samples, two-tailed unpaired t-test). Control: untreated healthy mice, UUO model: unilateral ureteral obstruction model mice, folic acid model: mice treated with folic acid to induce kidney injury, adenine model: mice treated with adenine to induce kidney injury, LTL lotus tetragonolobus lectin. Source data are provided as a Source Data file.

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