Fig. 5: In vivo treatment efficacy of TP/BIBF-bHDL in UUO model and folic acid model mice. | Nature Communications

Fig. 5: In vivo treatment efficacy of TP/BIBF-bHDL in UUO model and folic acid model mice.

From: High-density lipoprotein nanoparticles spontaneously target to damaged renal tubules and alleviate renal fibrosis by remodeling the fibrotic niches

Fig. 5

a Scheme of UUO model and administration regimen. b–d HE (b), Masson staining (c) and TUNEL assay (d) of kidney tissues of UUO mice after different treatments. Scale bars, 50 μm. n = 3 biologically independent samples. e, f The injury score (e) and fibrosis area (f) of kidney of UUO mice after different treatments. Data are mean ± SD (n = 3 biologically independent samples, one-way ANOVA with Dunnett’s multiple comparisons test and adjustment applied). g Western blot analysis of α-SMA expression in kidney of UUO mice after different treatments. n = 3 biologically independent samples. h Description of folic acid model and administration regimen. i–k HE (i), Masson staining (j) and TUNEL assay (k) of kidney tissues from folic acid-induced mice after different treatments. Scale bars, 50 μm. n = 3 biologically independent samples. l, m The injury score (l) and fibrosis area (m) of kidney of folic acid model mice after treatment. Data are mean ± SD (n = 3 biologically independent samples, one-way ANOVA with Dunnett’s multiple comparisons test and adjustment applied). n Western blot analysis of α-SMA expression in kidney of folic acid-induced mice after different treatments. n = 3 biologically independent samples. o, p Semi-quantification of α-SMA protein expression in UUO model mice and folic acid model mice with different treatments by Image J based the western blots shown in g (o) and n (p). It was normalized with corresponding α-tubulin signal. α-tubulin was used as internal control. The value of 42 and 55 kDa are referred to the predicted protein weight of α-SMA and α-tubulin. Data are mean ± SD (n = 3 biologically independent samples, one-way ANOVA with Dunnett’s multiple comparisons test and adjustment applied). a, h created in BioRender. He, S. (2024) https://BioRender.com/k11e324. UUO model unilateral ureteral obstruction model mice, control healthy mice without any treatment, PBS Phosphate buffered saline, TP/BIBF mixed solution of triptolide and nintedanib, TP/BIBF-bHDL triptolide and nintedanib co-loaded biomimetic high-density lipoprotein nanoparticles, HE hematoxylin-eosin staining, TUNEL Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick-End Labeling. Source data are listed in the Source Data file.

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