Fig. 3: Involvement of Pdr1 and Cdr1 in azole resistance of the C. glabrata ipi1^R70H mutant.

a Effects of CDR1 and PDR1 deletion on azole susceptibility were examined using a spot dilution assay in the wild-type and ipi1^R70H backgrounds. Logarithmic-phase cells of the C. glabrata strains were serially diluted and spotted on SC plates containing fluconazole or voriconazole at the indicated concentrations. Plates were incubated at 30 °C for 2 days. b Pdr1 P927S, which is known as a gain-of-function mutation in C. glabrata, was introduced in the wild-type and ipi1^R70H backgrounds. A spot dilution assay was performed as described above except that plates were incubated at 37  °C. All susceptibility tests were performed on at least three separate occasions.