Fig. 4: Ssb and Ssz1 physically interact with Ipi1, Pdr1, and each other. | Nature Communications

Fig. 4: Ssb and Ssz1 physically interact with Ipi1, Pdr1, and each other.

From: Mechanisms of multidrug resistance caused by an Ipi1 mutation in the fungal pathogen Candida glabrata

Fig. 4

Each protein C-terminally tagged with three tandem repeats of FLAG (3 × FLAG) or HA was expressed. Logarithmic-phase cells were lysed, and total protein was extracted. FLAG-tagged proteins were immunoprecipitated (IP) using an anti-FLAG antibody. Proteins were separated by SDS-PAGE, and immunoblotted (IB) using anti-FLAG or HA antibodies. a Ipi1-3×FLAG and Ssb-HA, (b) Ipi1-3 × FLAG and Ssz1-HA, (c) Ssb-3 × FLAG and Ssz1-HA, (d) Pdr1-3×FLAG and Ssb-HA, and (e) Pdr1-3 × FLAG and Ssz1-HA were analyzed. These experiments were repeated twice with similar results. Source data for all panels, including uncropped and unprocessed scans with molecular weight markers, are provided as a Source Data file.

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