Fig. 5: Effects of SSB1, SSB2 and SSZ1 deletions on antifungal susceptibility in the wild-type and ipi1R70H backgrounds. | Nature Communications

Fig. 5: Effects of SSB1, SSB2 and SSZ1 deletions on antifungal susceptibility in the wild-type and ipi1R70H backgrounds.

From: Mechanisms of multidrug resistance caused by an Ipi1 mutation in the fungal pathogen Candida glabrata

Fig. 5

a–d Logarithmic-phase C. glabrata cells were serially 10-fold diluted and spotted onto SC plates containing micafungin, fluconazole, or voriconazole at the indicated concentrations. Plates were incubated at 30 °C for 96 h. e, f Expression levels of CDR1, CDR2, and PDR1 are elevated in the ssb∆ and ssz1∆ strains. qRT-PCR was performed as described in the methods section and in Fig. 2b. Data are expressed as the means ± standard deviations (*P < 0.05; ***P < 0.001; ****P < 0.0001, ordinary one-way ANOVA with Dunnett’s multiple comparison test). qRT-PCR was repeated on three independent occasions with similar results. Source data for panels (e) and (f) are provided as a Source Data file.

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