Fig. 8: Effects of loss of calcineurin on antifungal susceptibility of the C. glabrata ipi1R70H mutant.
a Either calcineurin catalytic subunit A (CNA1) or regulatory subunit B (CNB1) was deleted in the wild-type and ipi1R70H backgrounds, and the antifungal susceptibility was examined using a spot dilution assay. Logarithmic-phase cells of the C. glabrata strains were serially diluted and spotted on SC plates containing an antifungal agent at the indicated concentrations. Plates were incubated at 37 °C for 2 days. b Expression level of FKS2 in the presence and absence of micafungin was determined by qRT-PCR. Logarithmic-phase cells of the C. glabrata strains were exposed to 1 µg/mL of micafungin in SC broth at 37 °C for 1 h. qRT-PCR was performed as described in the materials and methods and in Fig. 2b. Individual dots are shown for biological replicates (n = 6, each). The means ± standard deviations are shown (ns, not significant; Kruskal-Wallis test with Dunnett’s multiple comparison test). c C. glabrata cell survival was assessed before and after exposure to micafungin. Logarithmic-phase cells of the C. glabrata strains were exposed to 0.2 µg/mL of micafungin in SC broth at 37 °C. The CFUs were determined at the indicated time points, and percent CFU was calculated relative to the CFU prior to micafungin exposure. Data are presented as mean values ± standard deviations. The experiments shown in panels (a–c) were repeated on three independent occasions with similar results. Source data for panels (b) and (c) are provided as a Source Data file.
