Fig. 1: Modified Cas9s developed in this study and their in vitro DNA cleavage efficiencies. | Nature Communications

Fig. 1: Modified Cas9s developed in this study and their in vitro DNA cleavage efficiencies.

From: Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Fig. 1

a Cas9 modification scheme and proposed differential binding effects on the on- and off-target DNAs. b Representation of the wild-type and various modified Cas9s. c The 1.3 kb double stranded DNAs showing the specified target sequences used for in vitro cleavage reactions. d Cleavage efficiencies of various modified Cas9s on target DNAs. The efficiency of reactions was measured after 30 min of reaction and visualized using agarose gel electrophoresis. All reactions were performed in triplicates (n = 3). The mean was plotted with error bar representing standard deviation. P values were determined by unpaired two-tailed Student’s t-test based on cleavage efficiency of each protein with on-target linear DNA (ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). Source data are provided as a Source Data file.

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