Fig. 5: Capturing of FK506 BGC and production of FK506 in S. coelicolor M1146. | Nature Communications

Fig. 5: Capturing of FK506 BGC and production of FK506 in S. coelicolor M1146.

From: Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Fig. 5

a The scheme for capturing, cloning, and refactoring large BGC to produce FK506 in S. coelicolor M1146. b HPLC plots of different strains harvested 8 d after cultivation. Metabolite peaks shown in both S. tsukubaensis NRRL 18488 and S. coelicolor-FKKN strains are pointed by dotted gray lines. FK506 peak is highlighted by a star. c FK506 production during flask cultivation of different strains was presented. All cultivations were performed in triplicates (n = 3). The mean was plotted with error bar representing standard deviation. P values were determined by unpaired two-tailed Student’s t-test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Source data are provided as a Source Data file.

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