Fig. 6: Measurement of toxicity and inhibition efficiency of dCas9 and dCas9-BD in S. coelicolor M1146. | Nature Communications

Fig. 6: Measurement of toxicity and inhibition efficiency of dCas9 and dCas9-BD in S. coelicolor M1146.

From: Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Fig. 6

a Cell growth measured by phenylamine staining and (b) Expression of the gusA gene measured by GUS assay at 48 h after cultivation. The results of the strain without dCas9 and sgRNA are represented in black columns. Strains expressing wild-type dCas9 and dCas9-BD are represented in red and blue, respectively. Expressed sgRNA listed as C describes the absence of both dCas9 and sgRNA; 0 represents dCas9 without sgRNA; 1 describes the sgRNA targeted promoter region of the gusA gene; 2 describes the sgRNA targeted 5’ region of a gusA gene; 3 describes the sgRNA targeting the middle of a gusA gene; and 3 g describes expressing all three sgRNAs (1-3). All cultivations were performed in triplicates (n = 3). The mean was plotted with an error bar representing the standard deviation. P values were determined by unpaired two-tailed Student’s t-test with results obtained from strain lacking both dCas9 and sgRNA (ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). Source data are provided as a Source Data file.

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