Fig. 7: Secondary metabolite production in S. coelicolor A3(2) via utilization of dCas9-BD with multiple sgRNAs. | Nature Communications

Fig. 7: Secondary metabolite production in S. coelicolor A3(2) via utilization of dCas9-BD with multiple sgRNAs.

From: Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Fig. 7

a The central metabolic pathway involved in the secondary metabolite production of S. coelicolor A3(2). The malonyl-CoA and acetyl-CoA highlighted in blue are precursors of target metabolites, actinorhodin, undecylprodigiosin, and coelimycin P2. The genes highlighted in red, pyc, gltA, adhE, fabD, and fabH, present the inhibition targets to achieve precursor accumulation. b The secondary metabolites actinorhodin, prodigiosin, and coelimycin P2, were produced by the 23 strains engineered using dCas9-BD. The five strains showing a dramatic improvement in secondary metabolite production are marked with blue arrows. c ACT, actinorhodin; d RED, undecylprodigiosin; e CPK P2, coelimycin P2 productions of the five strains in a flask cultivation; WT, S. coelicolor A3(2); WT*, S. coelicolor A3(2) harboring pdCIRSPomyces-2BD without sgRNA; S. coelicolor A3(2)*, S. coelicolor A3(2) harboring pdCRISPomyces-2BD without sgRNA. f The harbored sgRNAs in five high-producing strains. Flask cultivations were performed in triplicates (n = 3). The mean was plotted with an error bar representing the standard deviation. Source data are provided as a Source Data file.

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