Fig. 1: Design and performance of the suppression drive HSDdsx.
From: Gene drive-based population suppression in the malaria vector Anopheles stephensi
A HSDdsx is inserted into the female-specific intron 4-exon 5 boundaries of dsx. The drive element contains a nanos-Cas9 cassette, a 3xP3-EGFP-SV40 marker, and two gRNAs under the control of U6A and 7SK promoters. B A drive allele will express Cas9 to cut the wild-type allele in the germline, after which the wild-type allele is either converted to drive allele or disrupted via end-joining. Maternally deposited Cas9/gRNA can cut wild-type alleles in the embryo, followed by disruption through end-joining. Females carrying two nonfunctional alleles (drive or resistance) will be sterile. C Drive heterozygotes were outcrossed with wild-type, and their offspring were phenotyped for EGFP fluorescence, indicating the rate of drive inheritance. The size of the dots indicates the relative sample size of larval progeny from a single cross batch. “n” indicates the total number of offspring in each group. The mean and standard error of the mean (SEM) are displayed.
