Fig. 4: Cross scheme of HSDdsx and vasa-Cas9 lines and the drive inheritance in offspring.
From: Gene drive-based population suppression in the malaria vector Anopheles stephensi
A HSDdsx females and vasa-Cas9 males were crossed to generate double heterozygous males, which were crossed to wild-type for assessing drive inheritance in offspring. Disrupted dsx can come from drive conversion and/or resistance allele formation in somatic cells. B The left panel picture was taken with an EGFP filter, and the right panel was under white light. Triangles with different colors show the differences in the fluorescence patterns between the lines. Wild-type (WT) mosquitoes are also displayed for comparison. C In the offspring of double heterozygous drive males and wild-type females, HSDdsx and vasa-Cas9 inheritance rates were measured.
