Fig. 7: APT2 ablation aggravates colon cancer development.
From: Palmitoylation-dependent regulation of GPX4 suppresses ferroptosis
a Timeline of the AOM/DSS-induced colon cancer model in C57BL/6J transgenic mice. b Representative images of large intestine tumorigenesis in Vil1-Cre; APT2+/+, Vil1-Cre; APT2flox/+, and Vil1-Cre; APT2flox/flox mice. Scale bar, 10 μm. c Quantification of tumor numbers in the large intestine of Vil1-Cre; APT2+/+, Vil1-Cre; APT2flox/+, and Vil1-Cre; APT2flox/flox mice. d H&E staining of colorectal lesions induced by AOM/DSS in Vil1-Cre; APT2+/+, Vil1-Cre; APT2flox/+, and Vil1-Cre; APT2flox/flox mice. Scale bar, 1 mm and 100 μm. e IHC staining for APT2, GPX4, and 4-HNE in colorectal lesions induced by AOM/DSS in Vil1-Cre; APT2+/+, Vil1-Cre; APT2flox/+, and Vil1-Cre; APT2flox/flox mice. Scale bar, 10 μm. f Intensity scoring of 4-HNE staining in colorectal lesions induced by AOM/DSS in Vil1-Cre; APT2+/+, Vil1-Cre; APT2flox/+, and Vil1-Cre; APT2flox/flox mice. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with Tukey’s test for (c, f). Sample sizes: (c, f) n = 6 independent tumors. Image in d, e shows representative results from three independent experiments.
