Fig. 3: Maximum readthrough in Induro-tRNAseq.
From: Genome-wide profiling of tRNA modifications by Induro-tRNAseq reveals coordinated changes
a The L-shaped tRNA structure showing RT-readable modifications. b Yield of readthrough (%) in end-to-end cDNA synthesis at 25, 37, 42, and 55 °C after the Induro RT reaction for 1, 2, or 16 h. Data were collected from total RNA of K562 cells as the input (n = 2; technical replicates). Center line: median; box limits: upper and lower quartiles; whiskers: 1.5X interquartile range; points: outliers. N.D.; Not detectable. **p < 0.01; two-tailed unpaired t-test. c Heatmaps of the frequency (%) of the RT misincorporations and RT stops at the annotated modifications. d Left: A scatter plot showing the frequency (%) of the RT misincorporation as a function of the G9:m1G9 ratio of the transcript of human mt-Leu(TAA) (n = 2; technical replicates). Right: The frequency of misincorporation of the native mt-Leu(TAA) in sequencing analysis of a total RNA sample. Data were collected from K562 cells with total RNA as the input under the RT reaction at 42 °C for 16 h (n = 2; technical replicates). Source data containing p values and n values are provided in Source Data 2.
