Fig. 2: 7SL target genes are suppressed by heat shock.

a Heatmap shows the intensity of 7SL CHART-seq signal over the binding site pre- and post-HS. The indicated region represents the peak center −/+ 3 kb. b Gene Ontology analysis (Biological process)⁴⁵ was performed for 7SL CHART-Seq peaks detected in both replicates in pre- or post-HS conditions. −Log₁₀ Benjamini P value is indicated for each term. Benjamini P value is adjusted p value for the multiple testing corrections. Source data are provided as a Source Data file. c Genome browser (IGV) view of 7SL CHART-Seq signal covering a region of the Btf3 gene. Tracks represent the Antisense (AS) signal subtracted for Sense (S) signal for two biological replicates pre- and post-HS. d Genome browser (IGV) view of 7SL CHART-Seq signal covering a region of the Med4 gene. Tracks represent the Antisense (AS) signal subtracted for Sense (S) signal for two biological replicates pre- and post-HS. e Genome browser (IGV) view of 7SL CHART-Seq signal covering a region of the pre-ribosomal Rn45s gene. Tracks represent the Antisense (AS) signal subtracted for Sense (S) signal for two biological replicates pre- and post-HS. f RT-qPCR analysis of the DNA eluate following 7SL CHART pre- and post-HS. For each indicated genomic sequence the enrichment ratio was calculated between the Antisense (AS) and Sense (S) 7SL probe. Data were presented as mean values ± SD. Source data are provided as a Source Data file. g Analysis of PRO-Seq expression patterns of 7SL target genes 1 h after exposure to heat shock (42 °C). Distribution of the entire PRO-Seq data set (blue) was compared to distribution of 7SL RNA-target genes (orange). Four different gene groups are shown based on the type of transcriptional regulation: downregulated (DownReg), unexpressed (UnExp), unregulated (UnReg), or upregulated (UpReg) genes. Source data are provided as a Source Data file. h Cumulative distribution function (CDF) of the Log₂ fold change following exposure to heat shock for the entire PRO-Seq dataset (black line) and for 7SL RNA-target genes (red line). P value is calculated using Kolmogorov–Smirnov (KS), a two-sided test. Source data are provided as a Source Data file.