Fig. 4: 3D stress distribution of a spherical indentation on soft materials.

a Scheme of three-dimensional (3D) compression tests. b Fluorescence images of a chitin-based hydrogel under spherical indentation from side views. Scale bar, 50 μm. All measurements were carried out in triplicate. c Split three-dimensional fluorescence images of an FTSM-CBM-1-labeled hydrogel. d 3D stress distribution via finite element analysis (FEA) under spherical indentation, with a modulus of 1.5 MPa. e Stress distribution curves from FEA in (d). Solid and dotted lines representing data from FEA and FTSM-CBM, respectively. f Top-view snapshots of each layer from FEA. The LUT showing the von Mises distribution.