Fig. 1: Specificity of IAJD34 for targeted mRNA delivery to the lung.
IAJD34 formulated with luciferase mRNA (Luc mRNA-IAJD34) was i.v. injected into BALB/c mice and analyzed for efficient pulmonary mRNA using IVIS. A The chemical structure of one-component IAJD3440. B Specificity of Luc mRNA-IAJD34 delivery to the lung. Representative IVIS images of whole-body and organs of mice at 4 h post-injection of 10 μg Luc mRNA-IAJD34 show luminescent biodistribution of luciferase expression predominantly in the lungs. IVIS images were analyzed, and the photon radiance (s−1sr−1m−2) of each image was quantified. The quantitative bioluminescence imaging (BLI) data are shown as total flux (p/s). C Time course luciferase expression in vivo. Representative IVIS images of whole-body mice following 10 μg Luc mRNA-IAJD34, imaged at 4, 24, 48, or 72 h post-injection. D Whole-body radiant flux (p/s) at various time points following the injection of 10 μg Luc mRNA-IAJD34. Data presented as mean ± SE, with n = 12 for 4 h, n = 15 for 24 h, n = 18 for 48 h, and n = 6 for 72 h. E Dose-dependent pulmonary delivery of Luc mRNA-IAJD34. Representative IVIS images of whole-body and organs of mice injected with Luc mRNA-IAJD34 at doses of 10, 20, or 30 μg, imaged at 24 h post-injection. Particle size (nm) and polydispersity index (PDI) for Luc mRNA-IAJD34 at each dose were measured prior to injection and are shown above the corresponding mouse IVIS image. F Delivery of Luc mRNA-IAJD34 to the lung parenchyma. At 24 h post-injection with 30 μg Luc mRNA-IAJD34, lung tissue was harvested and immunohistochemistry (IHC) for luciferase or IgG was performed and compared to naïve animals. Experiment was repeated 2 independent times. Scale bar = 100 µm. Black = Naïve, Red = 10 μg, Blue = 20 μg, Green = 30 μg. Source data are provided as a Source Data file.
