Fig. 10: Effects of TGF-β mRNA-IAJD34 bleomycin-induced cytokine production.

BALB/c mice were exposed to PBS or bleomycin (ITB) and subsequently received either an empty IAJD34 or 10 µg TGF-β mRNA-IAJD34. Cell-free BAL fluid was collected 3 days post-exposure and injection. A–E Cell-free BAL fluid was evaluated for cytokines using a Milliplex Max Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay. The concentrations of (A) G-SCF, (B) IL-6, (C) CXCL10, (D) IL-1α, and (E) IL-2 are presented as pg/mL. Date shown a mean ± SE, n/group = 7/PBS + Empty-IAJD34, 6/PBS + TGF-β mRNA + IAJD34, 10/ITB + Empty-IAJD34, 8/ITB + TGF-β mRNA+IAJD34. Data were evaluated for normality using a Shapiro Wilk’s test. If data were normally distributed, they were compared using a 2-way ANOVA followed by Šídák’s multiple comparisons test. Data points that were identified as 0 were set to the limit of detection according to manufacture values for statistical analysis. If data were not normally distributed, they were evaluated using a Kruskal Wallis test with Dunn’s multiple comparison test. * indicates a significant difference, p < 0.05. ((A): PBS/Empty-IAJD34 vs ITB/Empty-IAJD34 p = 0.0231, ITB/Empty-IAJD34 vs ITB/TGF-β mRNA-IAJD34 p = 0.0300; (B): PBS/Empty-IAJD34 vs ITB/Empty-IAJD34 p = 0.0057, ITB/Empty-IAJD34 vs ITB/TGF-β mRNA-IAJD34 p = 0.0285; (C): PBS/Empty-IAJD34 vs ITB/Empty-IAJD34 p = 0.0168; (D): PBS/Empty-IAJD34 vs ITB/Empty-IAJD34 p = 0.0296; (E) PBS vs ITB p = 0.0107). Source data are provided as a Source Data file.