Fig. 4: Dose-dependent expression of TGF-β mRNA-IAJD34 in lung tissue. | Nature Communications

Fig. 4: Dose-dependent expression of TGF-β mRNA-IAJD34 in lung tissue.

From: Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers

Fig. 4

Various doses of TGF-β mRNA-IAJD34 (10, 20, and 30 μg per mouse) were delivered to BALB/c mice, and lung tissue was collected 24 h post-injection and analyzed for TGF-β protein expression. A Western blots for TGF-β were performed on homogenized whole lung tissue at indicated time points. Representative images are shown. B Western blots results were quantified using densitometry. TGF-β protein expression was normalized to β-actin for each band, and the density was expressed relative to the naïve control. Data are presented as mean ± SE for n = 4–9 per group. Statistical comparisons were made using a two-tailed Mann-Whitney U test where * indicates a significant difference from the control, p < 0.05. ((B) Unprocessed TGF-β: Naïve vs 20 μg p = 0.0012, Naïve vs 30 μg p = 0.0046; Cleaved TGF-β: Naïve vs 10 μg p = 0.0557, Naïve vs 20 μg p = 0.0029, Naïve vs 30 μg p = 0.0026; Total TGF-β: Naïve vs 20 μg p = 0.0003, Naïve vs 30 μg p = 0.0444). Source data are provided as a Source Data file.

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