Fig. 7: TGF-β protein expression is transiently induced after mRNA delivery to lung via IAJD34. | Nature Communications

Fig. 7: TGF-β protein expression is transiently induced after mRNA delivery to lung via IAJD34.

From: Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers

Fig. 7

Empty IAJD34 or 10 ug TGF-β mRNA-IAJD34 were delivered to BALB/c mice, and lung tissue homogenates were evaluated for TGF-β protein expression at 4, 24, and 48 h post-injection. A Western blots for TGF-β were performed on homogenized whole lung tissue. Representative images are shown. B Western blots bands were quantified by densitometry and TGF-β protein expression was normalized to β-actin for each band, and the density was expressed relative to the naïve control. Data are presented as mean ± SE, n = 3–5 per group. Statistical comparisons were made using a two-tailed Mann-Whitney U test, where * indicates a significant difference from the matched empty control, p < 0.05. ((B) Unprocessed TGF-β: Control vs 4 hr p = 0.0357; Cleaved TGF-β: Control vs 4 hr p = 0.0357, Control vs 24 hr p = 0.0159; Total TGF-β: Control vs 4 hr p = 0.0357). Source data are provided as a Source Data file.

Back to article page