Fig. 8: Effects of TGF-β mRNA-IAJD34 on bleomycin-induced histological alterations. | Nature Communications

Fig. 8: Effects of TGF-β mRNA-IAJD34 on bleomycin-induced histological alterations.

From: Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers

Fig. 8

BALB/c mice were exposed to PBS or bleomycin (ITB) and subsequently received either an empty IAJD34 or 10 µg TGF-β mRNA-IAJD34. Lung tissue was collected 3 days post-exposure and injection. Fixed lung tissue was processed and stained with H&E. Representative H&E images are shown. The slides are shown at 400x magnification, with scale bars indicating 50 µm. An inset image shows the data of evaluating alveolar thickness, where 10 fields per slide were randomly selected and analyzed using ImageJ. Data are presented as Median ± SE, n = 6–10 per group. Statistical comparisons were made using a two-tailed Wilcoxon ranked sum test, where * indicates a significant difference from the PBS/empty-IAJD34 group, p < 0.05. (Alveolar Thickness: PBS/Empty-IAJD34 vs ITB/Empty-AJD34 p = 0.0273, PBS/Empty-IAJD34 vs PBS/TGF-β mRNA-IAJD34 p = 0.0625, ITB/Empty-IAJD34 vs ITB/TGF-β mRNA-IAJD34 p = 0.2188). Scale bar = 50 µm. Source data are provided as a Source Data file.

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