Fig. 5: FLAD1 promotes de novo lipogenesis via the upregulation of lipogenic enzymes. | Nature Communications

Fig. 5: FLAD1 promotes de novo lipogenesis via the upregulation of lipogenic enzymes.

From: Copy number amplification of FLAD1 promotes the progression of triple-negative breast cancer through lipid metabolism

Fig. 5

a, b Schematic diagrams of fatty acid (a) and cholesterol (b) metabolic processes. c Relative mRNA levels of the indicated fatty acid and cholesterol biosynthesis genes following shRNA-mediated knockdown of FLAD1 in LM2-4175 and SUM159 cells. d Relative mRNA levels of the indicated fatty acid and cholesterol biosynthesis genes following the overexpression of WT FLAD1 and FLAD1-R530C in HCC1806 and Hs578T cells. e Relative protein levels of the indicated fatty acid and cholesterol biosynthesis genes following shRNA-mediated knockdown of FLAD1 in LM2-4175 and SUM159 cells. f Relative protein levels of the indicated fatty acid and cholesterol biosynthesis genes following the overexpression of WT FLAD1 and FLAD1-R530C in HCC1806 and Hs578T cells. g The concentrations of FASN and ACC1 were measured in control and FLAD1 knockdown SUM159 cells. h The concentrations of FASN and ACC1 were measured in Hs578T cells overexpressing the control vector, WT FLAD1 or FLAD1-R530C. Graph bars represent mean ± SEM, and P value was calculated by two-tailed Student’s t-test. Results in (c, d, g, h) represent biologically independent experiments of n = 3. e, f n = 3 independent experiments, a representative example is shown. Source data are provided as a Source Data file.

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