Fig. 2: VSTM2L is positively associated with prostate cancer aggressiveness. | Nature Communications

Fig. 2: VSTM2L is positively associated with prostate cancer aggressiveness.

From: VSTM2L protects prostate cancer cells against ferroptosis via inhibiting VDAC1 oligomerization and maintaining mitochondria homeostasis

Fig. 2

A The top (1-25) over-expressed genes in Prostate adenocarcinoma (PRAD) were analyzed based on the TCGA cohort by using the UALCAN database (Normal, n = 52; Tumor, n = 497). B The mRNA levels of VSTM2L in PRAD and normal tissues of TCGA PRAD cohort and GTEX prostate normal data. P = 7.168E-52. C The mRNA levels of VSTM2L in PRAD and normal tissues from GENT2 database. P = 3.2305E-7. D, E The protein levels of VSTM2L from 78 paired clinical prostate cancer specimens. The IHC staining score was used to quantify the protein levels of VSTM2L. Scale bar =250 μm. P = 2.7147E-17. F, G Association between Progression-free-survival (F) or Disease-free survival (G) of prostate cancer patients and VSTM2L mRNA expression from the TCGA database. H, I The cell proliferation was measured in VSTM2L knockdown 22Rv1 (Control VS shVSTM2L-1 P = 0.000045, Control VS shVSTM2L-2 P = 2.1828E-8) (H) or DU145 (Control VS shVSTM2L-1 P = 0.000002, Control VS shVSTM2L-2 P = 0.000011) (I) cells by CCK8 assay. Data are shown as the mean ± SD (n = 5 biological replicates). J, K The effects of VSTM2L knockdown on the growth of 22Rv1 (Control VS shVSTM2L-1 P = 4.3015E-7, Control VS shVSTM2L-2 P = 4.1099E-7) or DU145 (Control VS shVSTM2L-1 P = 2.9544E-7, Control VS shVSTM2L-2 P = 8.3674E-8) cells, as detected using the colony formation assay. Data are shown as the mean ± SD (n = 4 biological replicates). L-N Wound healing analysis for assessing migration of the indicated cell strains at 0 h and indicated endpoint. Representative images (L, M) and quantification (N) are shown as indicated. Data are presented as the mean ± SD (n = 3 biological replicates). Scale bar = 200 and 250 μm. O, P Representative pictures (O) and quantification analysis (P) of migration assays in control and VSTM2L knockdown 22Rv1 cells (Control VS shVSTM2L-1 P = 0.000008, Control VS shVSTM2L-2 P = 0.000007) or DU145 cells. Data are plotted as mean ± SD (n = 3 biological replicates). Scale bar = 250 μm. P value was determined by unpaired two-tailed Student’s t-test without adjustments. (B, C), paired two-tailed Student’s t-test without adjustments (E), log-rank test (F, G), two-way ANOVA (H, I) and one-way ANOVA (K, N, P). Source data are provided as a Source Data file.

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