Fig. 1: The formation of new bone relies heavily on the crucial involvement of blood clots. | Nature Communications

Fig. 1: The formation of new bone relies heavily on the crucial involvement of blood clots.

From: In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment

Fig. 1

A Meta analysis using specific keywords related to osteogenesis and blood clots. B Comparison of coagulation time and blood loss after tooth extraction in Ctrl and BCD group (rats). (n = 6 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). C, D Fibrin staining and quantification D of blood clot at 3 h, 6 h, 24 h, and one week post tooth extraction. (n = 6 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test, p < 0.0001). E Masson’s trichrome and VG staining of extraction region from Ctrl and BCD group at 1 week post tooth extraction. F Micro-CT analysis of extraction region from Ctrl and BCD group. G The quantification of Micro-CT analysis, including bone mineral density (BMD), bone volume to total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp). (n = 6 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). H, I Immunofluorescent staining and quantification (I) of Runx2 and Alp. (n = 6 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). J, K The immunofluorescent staining of CD73 and CD105 was analyzed using a Confocal Laser Scanning Microscope (CLSM), and the quantification of double positive signals is presented in (K). (n = 6 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test).

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