Fig. 2: The formation of blood clots promotes osteogenesis by enhancing Integrin-mediated focal adhesion signaling. | Nature Communications

Fig. 2: The formation of blood clots promotes osteogenesis by enhancing Integrin-mediated focal adhesion signaling.

From: In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment

Fig. 2

A t-Distributed Stochastic Neighbor Embedding (t-SNE) plots of broad cell types within the socket and surrounding tissue in both the Ctrl and BCD groups at 24 h post tooth extraction. (n = 3 independent replicates). B The signature gene expression in each cluster served as the criterion for clustering. C The osteogenic cells were further classified into four sub-clusters based on the dynamic changes of four genes, namely CD73, CD105, Alpl, and Ocn. D The proportion of the sub-cluster within osteogenic cells in quasi-temporal analysis. (n = 3 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test, ***p < 0.001). E The expressions of osteogenic-related genes in the Ctrl group and BCD group. (n = 3 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). F–I A comparative single-cell Gene Set Enrichment Analysis (sc-GSEA) were conducted on the four sub-clusters to identify the specific signaling pathways between Ctrl and BCD groups. The GSEA p-value represents the estimation of significance. We evaluate the significance of an observed Enrichment Score (ES) by comparing it to a distribution of ESNULL scores derived from randomly assigned phenotypes.

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