Fig. 7: BCgel potently enhances osteogenesis in the blood clot defect (BCD) beagle model.

A Photos of beagle tooth extraction and the comparison of coagulation time in gelform (Ctrl) and BCgel treated immediately after tooth extraction. (n = 7, including 4 beagles for 2 months treatment and 3 beagles for 5 months treatment, means ± SD, p value calculated by two-tailed Student’s t test). B, C Micro-CT analysis and quantitative results of Ctrl and BCgel groups after 2 months of treatment. (n = 4 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). D H&E, Masson’s trichrome and VG staining of extraction region from Ctrl and BCgel group at 2 months post treatment. E–I Ctrl and BCgel groups were analyzed for immunotoxicity (E), hematotoxicity (F), hepatotoxicity (H), and nephrotoxicity (I). H&E staining of organs in the BCgel group after 2 months of treatment (G). (n = 4 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). J, K Micro-CT analysis and quantitative results of the Ctrl and BCgel groups were assessed after 5 months of treatment. (n = 3 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test). L H&E, Masson’s trichrome and VG staining of extraction region from Ctrl and BCgel group at 5 months post treatment. M–P)Ctrl and BCgel groups were analyzed for hematotoxicity (M), hepatotoxicity (N), and nephrotoxicity (P). H&E staining of organs in the BCgel group after 5 months of treatment (O). (n = 3 independent replicates, means ± SD, p value calculated by two-tailed Student’s t test).