Fig. 2: Non-canonical lysosomal lipolytic mechanisms are functional with prolonged fasting.
From: Non-canonical lysosomal lipolysis drives mobilization of adipose tissue energy stores with fasting
a Schematic of adipocyte specific ATGL/Pnpla2 loss of function (KO) relative to control (FLOX), using mice that were 8–9 weeks old at study start. Paired blood draws were used to assess the lipolytic response to adrenergic stimulus (isoproterenol) and fasting. For panels d–h, data shown as mean, S.D.M. b Change in plasma NEFA with isoproterenol (ISO) or fast. Significance for genotype effect assessed by two-way ANOVA. FLOX male n = 5; KO male n = 7; FLOX female n = 5 with iso and n = 6 with fasting; KO female n = 7. c Change in plasma glycerol with isoproterenol (ISO) or fast. Significance for genotype effect assessed by two-way ANOVA. FLOX male n = 5; FLOX female n = 5; KO male n = 7; KO female n = 6. d Change in body weight assessed by two-tailed t-test: FLOX male n = 6; FLOX female n = 5; KO male n = 7; KO female n = 7. Significance assessed by two-tailed t-test. e Terminal plasma ketone levels. FLOX male n = 6; FLOX female n = 5; KO male n = 7; KO female n = 6. Significance assessed by two-tailed t-test. f Terminal plasma FGF21 levels. FLOX male n = 5; FLOX female n = 5; KO male n = 7; KO female n = 7. Significance assessed by two-tailed t-test. g Terminal plasma glucose levels. FLOX male n = 5; FLOX female n = 5; KO male n = 7; KO female n = 6. Significance assessed by two-tailed t-test. h qPCR of isolated inguinal (iWAT) and gonadal (gWAT) adipocytes for lipolysis and lysosome genes. FLOX n = 10 (male n = 5, female n = 5; KO n = 13 (male n = 7; female n = 6). Two-way ANOVA, **p = 0.008; ***p = 0.0002; ****p < 0.0001. i Immunoblots for iWAT and gWAT adipose tissue collected at the 24-hour fasting timepoint. Note: tubulin controls run on different gels.
