Fig. 4: Temporal transition from ATGL-dependent to LAL-dependent adipocyte lipolysis with fasting.
From: Non-canonical lysosomal lipolysis drives mobilization of adipose tissue energy stores with fasting
a ATGL loss of function (KO) by targeting Pnpla2 (b, d, e, h,i, l,m, p); LAL loss of function (KO) by targeting Lipa (c, f,g, j,k, n,o, q); each shown relative to controls (FLOX). For panels b–o, data shown as mean, S.D.M. b Isoproterenol (ISO)-stimulated non-esterified fatty acids (NEFA) (left) and glycerol (right). FLOX n = 12 (8 male, 4 female); KO n = 15 (8 male, 7 female). For b–g: two-way ANOVA, genotype effect. c ISO-stimulated NEFA (left) and glycerol (right). FLOX n = 19 (9 male, 10 female); KO n = 18 (10 male, 8 female). d Body weight change with fasting. FLOX n = 12 (8 male, 4 female); KO n = 13 (8 male, 5 female). e Plasma glucose change with fasting. FLOX n = 12 (8 male, 4 female); KO n = 13 (8 male, 5 female). f Body weight change with fasting. FLOX n = 19 (9 male, 10 female); KO n = 18 (10 male, 8 female). g Plasma glucose change with fasting. FLOX n = 19 (9 male, 10 female); KO n = 18 (10 male, 8 female). h Terminal plasma ketones. FLOX n = 12 (8 male, 4 female); KO n = 13 (8 male, 5 female). For h–k, significance assessed by two-tailed t-test. i Terminal liver triglycerides. FLOX n = 12 (8 male, 4 female); KO n = 13 (8 male, 5 female). j Terminal plasma ketones. FLOX n = 19 (9 male, 10 female); KO n = 18 (10 male, 8 female). k Terminal liver triglycerides. FLOX n = 19 (9 male, 10 female); KO n = 18 (10 male, 8 female). l qPCR of inguinal (iWAT) adipocytes from ATGL KO (n = 12) or control (n = 13). For l–o Two-way ANOVA: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. m qPCR of gonadal (gWAT) adipocytes: ATGL KO (n = 12) or FLOX (n = 13). n qPCR of inguinal adipocytes: LIPA KO (n = 12) or FLOX (n = 12). o qPCR of gonadal adipocytes: LIPA KO (n = 12) or FLOX (n = 12). p Immunoblot of inguinal and gonadal adipose tissues from control or adipocyte-specific ATGL loss of function (KO) mice; 24-hour fasting timepoint. q Immunoblot of inguinal and gonadal adipose tissues from control or LIPA loss of function (KO) mice; 24-hour fasting timepoint. Note for p,q: tubulin controls run on different gels.
