Fig. 6: FDN effects are intestine-specific. | Nature Communications

Fig. 6: FDN effects are intestine-specific.

From: An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice

Fig. 6

a H&E staining of the liver from acute UC mice. Scale bar = 100 μm. n = 3 mice, biological samples per group, a representative example is shown. b Liver-to-body weight ratios (liver index) in treated mice. Serum levels of alanine transaminase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were measured (n = 6 mice). c RT-qPCR analysis of PXR target gene expression cyp3a11 and cyp2b10 levels in liver (n = 6 mice). d RT-qPCR analysis of cyp3a4, cyp2b6, and ugt1a1 mRNA expression in PHH (from three donors) treated for 48 h with vehicle (Veh, 0.1% DMSO) or indicated concentrations of ligands (RIF and FDN at 10 μM; CITCO at 3 μM). Results were obtained from experiments performed in triplicate. b Data are shown as means ± SD, one-way ANOVA test, p-values compared to the DSS group. c, d Data were analyzed by two-way ANOVA with multiple comparisons and are expressed as mean ± SD. p-values compared to the DSS group (c) or the Veh (DMSO) group (d). RIF, rifampicin is a human PXR agonist. CITCO is a human CAR agonist. Veh vehicle, FDN furanodienone, PCN pregnenolone-16a carbonitrile, DSS dextran sodium sulfate. Source data are provided in the Source Data file.

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