Fig. 7: Exploring the adaptive evolution mechanism of PAO1 using HF-ID824-evoCDA-FrCas9n. | Nature Communications

Fig. 7: Exploring the adaptive evolution mechanism of PAO1 using HF-ID824-evoCDA-FrCas9n.

From: A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9

Fig. 7

a Schematic representation of the effects of multi-gene inactivation mediated by CBE on the adaptive evolution of PAO1 under sub-MIC PMB conditions. b HF-ID824-evoCDA-FrCas9n editing range on PAO1 genome. c Sanger sequencing results for the inactivation of six genes using HF-ID824-evoCDA-FrCas9n. d The MIC values of PAO1 and the six mutants were compared before exposure and after three days under 1/2 MIC PMB conditions (n = 8 biological replicates, data represent mean ± SD). Statistical significance was analyzed using a two-tailed t-test: ns, p > 0.05.

Back to article page