Fig. 2: Characterization of differentiated SC-derived islet cells and comparative analysis of islet niche.
From: Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets
a Schematic illustration of differentiation process and subsequent workflow for hESC-derived islet cells. Created in BioRender. Kim, M. (2024) https://BioRender.com/h44f786. b Brightfield images of stage 6 cells in 2D culture (top) and suspension culture (bottom) (scale bars, 500 µm). c Quantitative real time polymerase chain reaction (qPCR) analysis of endodermal and pancreatic genes at each differentiation stage. Forkhead box protein A2 (FOXA2), Pancreatic and duodenal homeobox 1 (PDX1), NK6 homeobox 1 (NKX6.1), Insulin (INS), Glucagon (GCG), Somatostatin (SST) (n = 3 samples for FOXA2, PDX1, NKX6.1, INS, n = 4 samples for GCG, SST, mean ± SD). Statistical significance was analyzed using one-way ANOVA with Dunnett’s multiple comparisons test: NS, not significant; PESC, S1 = 1.5 × 10−5, PESC, S2 = 2.2 × 10−6, PESC, S3 = 0.0131 (FOXA2); PESC, S2 = 0.9999, PESC, S3 = 0.5314, PESC, S4 = 0.1543 (PDX1); PESC, S2 = 0.9999, PESC, S3 = 0.9999, PESC, S4 = 3.3 × 10−5 (NKX6.1); PESC, S4 = 0.9999, PESC, S5 = 0.0743, PESC, S6 = 0.0023 (INS); PESC, S4 = 1, PESC, S5 = 0.9968, PESC, S6 = 0.1548 (GCG); PESC, S4 = 1, PESC, S5 = 0.1287, PESC, S6 = 7.1 × 10−5 (GCG) d Immunostaining for PDX1, NKX6.1, C-peptide, Chromogranin A (CHGA), GCG, SST of differentiated cells (scale bars, 50 µm). Three independent experiments were conducted to produce three different samples, and imaging was independently repeated with similar results. e Immunostaining for laminin (LAM), INS, collagen IV (COL IV) of human islets and SC-derived islets (scale bars, 100 µm). Three independent experiments were conducted to produce three different samples, and imaging was independently repeated with similar results. Source data are provided as a Source Data file.
