Fig. 5: H₂O₂-activated RNase L recruitment for pre-miR-21 degradation.

a Real-time HPLC profiling of RIBOTAC21-BA aqueous solutions in the presence of H₂O₂ (100 μM). b HPLC profiles of RIBOTAC21-BA in the presence of various species (1 mM), e.g. Na⁺, NaHS, GSH, SO₄^2-, NO₃^-, NOO^-, and H₂O₂. Representative PAGE gel images (c) and quantitative analysis (d) of the RNase L oligomerization induced by RIBOTAC21-BA or RIBOTAC21-BA + H₂O₂. n = 3 independent samples. Representative SDS-PAGE gel images (e) and quantitative analysis (f) of the degradation of pre-miR-21 under different treatments (the commercial pre-miR-21 was labeled by Cy3). n = 3 independent samples. g Diagram of the acidic pH/H₂O₂ dual-activated RiboTACs concept. Representative SDS-PAGE gel images (h) and (i) quantitative analysis of the pre-miR-21 degradation under different treatments. n = 3 independent samples. Data denote the mean ± standard deviation. Two-sided unpaired t-test. Source data are provided as a Source Data file.