Fig. 3: CD8⁺ T-cells are increased and CD206⁺ macrophages are decreased in the microenvironment of MC38 liver metastases following WNT11 knockdown.

a t-SNE plot illustrating the distribution of all CD45 + immune cells from liver metastases of MC38 cells with scramble control or WNT11 knockdown. b Proportion of differential cell clusters between MC38 scramble and shWnt11 liver metastases. c t-SNE plot of all CD45⁺ cells colored by the expression levels of CD206 in the scramble and shWnt11 group. d Comparison of CD206 expression of C02 between the scramble and shWnt11 group. e Comparison of I-A/I-E and CD24 expression of C16 between the scramble and shWnt11 group. f–i mIHC staining was used to verify the differences of I-A/I-E⁺CD8⁺ T-cells (f, g) and CD206⁺ macrophages (h, i) in MC38 liver metastases between the scramble and shWnt11 group. j CD206 expression of macrophages in MC38 liver metastases between the scramble and shWnt11 group detected using mIHC. k, l FACS analysis to determine the frequency of I-A/I-E + CD8 + T-cells relative to the total CD45 + T-cell population in MC38 liver metastases. m, n FACS analysis to determine the frequency of CD206 + F4/80 + macrophages relative to the total macrophages in MC38 liver metastases. b, g–j, l, n. P-values were calculated using a Student’s t test (two-tailed). Data are presented as the mean ± SD (n = 5 biological replicates). d, e P-values were calculated using a Wilcoxon rank-sum test (two-tailed). Source data are provided as a Source Data file.