Fig. 2: The number of GCN4 repeats in SunTag activators impacts gene activation efficiency.
From: Dynamic properties of transcriptional condensates modulate CRISPRa-mediated gene activation
a Schematic presenting various designs of CRISPR-SunTag activators. b Flow cytometry assessing the expression levels of the exogenous reporter (miniCMV-BFP) and showing the activation ratios of individual CRISPRa systems. c Fluorescent imaging analysis of miniCMV-BFP expression levels in single cells. n = 80 cells for each group, except for 10xVP (n = 29) and 3xVP (n = 81). Data are shown as mean ± SEM. d Schematic diagram using HSPB1 as an example to show the target locations of three sgRNAs upstream of the transcription start site (TTS). e, f Flow cytometry analysis evaluating the CRISPR activation ratios using either a mixture of three sgRNAs as shown in (e) or a single sgRNA (f). All data in (b, e), and (f) were analyzed from cells expressing transfection markers. Three biological replicates are displayed as mean ± SEM. Negative controls (NC) were performed by transfecting dCas9 lacking AD fusions and on-target sgRNAs. P values were calculated by one-way ANOVA test. Source data are provided as a Source Data file.
