Fig. 6: Aspirin promotes clearance of α-syn aggregates in a PD mouse model.

a Experimental scheme for establishing a PD mouse model and aspirin treatment. 10, 11, and 12 mice were used for the PBS, the PFF-control, and the PFF-aspirin treatments, respectively. The figure was created using BioRender.com. Open field test (b) and rotarod test (c) for PD mice. These behavioral tests were repeated twice during the aspirin administration. The results of the rotarod test were normalized to the batch-specific PBS mice in each independent experimental batch. Each dot represents a mouse (One way ANOVA with FDR correction for multiple comparisons, data presented as mean ± SEM). d Aspirin treatment decreases α-syn aggregates in the striatum. Representative immunofluorescence images (scale bar, 100 μm) and zoom-in merged images (scale bar, 10 μm) are shown. Quantitative results are shown in e–g, and the dot represents the mouse brain. Aspirin treatment reduces the intensity (e) and density (f) of α-syn aggregates in the striatum, which is represented by p-α-syn (n = 5 or 6, One way ANOVA with FDR correction for multiple comparisons, data presented as mean ± SEM). g DA neurons in the striatum with aspirin treatment (n = 5 or 6, One way ANOVA with Tukey’s multiple comparisons test). h Aspirin treatment increases the number of DA neurons in the substantia nigra. Representative immunofluorescence images (scale bar, 200 μm) and zoom-in merged images (scale bar, 20 μm) are shown. Quantitative results are shown in i–l, and the dot represents the mouse brain. Intensity (i) and density (j) of α-syn aggregates in the substantia nigra with aspirin treatment (n = 6, One way ANOVA with FDR correction for multiple comparisons, data presented as mean ± SEM). DA neurons in the substantia nigra with aspirin treatment (n = 6, One way ANOVA with FDR correction for multiple comparisons, data presented as mean ± SEM). m Aspirin treatment regulates ubiquitin linkages in mouse brains. The signature peptides representing seven types of ubiquitin linkages were used for quantification (n = 3, unpaired two-tailed t-test, data presented as mean ± SEM). Source data are provided as a Source Data file.