Fig. 8: Result of multiplex immunohistochemical analysis. | Nature Communications

Fig. 8: Result of multiplex immunohistochemical analysis.

From: Regorafenib plus sintilimab as a salvage treatment for microsatellite stable metastatic colorectal cancer: a single-arm, open-label, phase II clinical trial

Fig. 8

a, b Among all stains, DAPI (dark blue) for nucleus, panCK (light blue) for cancer cells, FoxP3 (red) for FoxP3 (+) Treg cells, CD103 (orange) for CD103 (+) T cells, CD68 (green) for CD68 (+) macrophages, PD‐L1 (purple) for PD-L1 (+) cells, CD20 (yellow) for CD20 (+) B cells, and CD8 (pink) for CD8 (+) T cells. Staining was performed only once in (a, b). c Comparison of cell density between treatment-sensitive (n = 15) and treatment-resistant groups (n = 15). d Cell distance within the tumor immune microenvironment. e Comparison of cell distance to CK+ cells between treatment-sensitive (n = 15) and treatment-resistant groups (n = 15). Centers, boxes, and whiskers indicate medians, quantiles, and minima/maxima, respectively, in (c, e), and two-sided Wilcoxon rank-sum test was used for comparison. Source data are provided as a Source Data file.

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