Fig. 2: ILF3 inhibits ZIKV replication and propagation via its dsRNA domain in human neuroblastoma cells and induced pluripotent stem cell (iPSC)-neural stem cell (NSC). | Nature Communications

Fig. 2: ILF3 inhibits ZIKV replication and propagation via its dsRNA domain in human neuroblastoma cells and induced pluripotent stem cell (iPSC)-neural stem cell (NSC).

From: Identification of antiviral RNAi regulators, ILF3/DHX9, recruit at ZIKV stem loop B to protect against ZIKV induced microcephaly

Fig. 2

A Western blot analysis of ILF3, ZIKV-E, NS5 and GAPDH protein expression in ZIKV-infected control or ILF3-knockdown SK-N-BE(2) cells (MOI = 1) at 48 hpi. n = 3 biological replicates/group. RT-qPCR analysis of ILF3 RNA expression (B) and ZIKV viral RNA copies (C) in ZIKV-infected control or ILF3-knockdown SK-N-BE(2) cells (MOI = 1) at 48 hpi. Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. ***p < 0.001. D Viral titers in ZIKV-infected control or ILF3-knockdown SK-N-BE(2) cells (MOI = 1) were quantified by plaque assay at 48 hpi. Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. ***p < 0.001. Immunofluorescence showing Flavivirus (E) and dsRNA (F) expression in ZIKV-infected control or ILF3-knockdown SK-N-BE(2) cells (MOI = 1) at 48 hpi (scale bar, 50 μm). Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. ***p < 0.001. G RT-qPCR analysis of ILF3 expression and viral RNA copies in ZIKV-infected control or ILF3-knockdown iPSC–NSC cells (MOI = 1) at 48 hpi. Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. **p < 0.01, ***p < 0.001. Immunofluorescence showing Flavivirus (H) and dsRNA (I) expression in ZIKV-infected control or ILF3-knockdown iPSC–NSC cell (MOI = 1) at 48 hpi (scale bar, 50 μm). Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. **p < 0.01. J Western blot analysis of ILF3, NS5, E, and GAPDH protein expression in ZIKV-infected control or ILF3 overexpressed SK-N-BE(2) cell (MOI = 1) at 48 hpi. n = 3 biological replicates/group. K RT-qPCR analysis of viral RNA copies in ZIKV-infected control or ILF3 overexpressed SK-N-BE(2) cell (MOI = 1) at 48 hpi. Bars, mean ± SD, n = 3 biological replicates/group, two-tailed Student’s t-test. *p < 0.05, **p < 0.01. L Western blot analysis of ILF3, NS5, E, and GAPDH protein expression in ZIKV-infected control or ILF3, NF90, NF110 WT or dsRNA-binding motif (dsRBM) mutant overexpressed SK-N-BE(2) cells (MOI = 1) at 48 hpi. n = 3 biological replicates/group. Source data are provided as a Source Data file.

Back to article page