Fig. 7: Treatment with vsiR-1 mimics inhibited ZIKV replication in vivo and protected mice from ZIKV-induced microcephaly. | Nature Communications

Fig. 7: Treatment with vsiR-1 mimics inhibited ZIKV replication in vivo and protected mice from ZIKV-induced microcephaly.

From: Identification of antiviral RNAi regulators, ILF3/DHX9, recruit at ZIKV stem loop B to protect against ZIKV induced microcephaly

Fig. 7

A Representative image of mock or ZIKV-infected WT or Nestin-Cre ILF3 cKO mice under scramble or vsiR-1 mimics treatment. Brain size (Middle) and brain weight (bottom) were captured at 7 dpi. Bars, mean ± SD, Mock group (n = 3 mice/group), ZIKV infection group (n = 5 mice/group), two-tailed Student’s t-test. *p < 0.05, **p < 0.01. Body weight change (B) and clinical score (C) were measured in mock or ZIKV-infected control or ILF3 cKO mice under scramble or vsiR-1 mimics treatment for 7 days. Bars, mean ± SD, Mock group (n = 3 mice/group), ZIKV infection group (n = 5 mice/group), one-way ANOVA analysis. *p < 0.05, **p < 0.01, ***p < 0.001. D HE staining of the hippocampus brain of indicated mice group (Scale bar, 100 μm). n = 3 mice. E Immunofluorescence showing the relative expression of GFP, SOX2, and ZIKV-E in the indicated mice treatment group. The immunofluorescence intensity was quantified by Image J software. Bars, mean ± SD, n  =  3 mice, two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. F Virus titers were quantified by virus plaque assay analysis from ZIKV-infected WT or ILF3-cKO mice under scramble or vsiR-1 mimics treatment. Bars, mean ± SD, n = 5 mice, two-tailed Student’s t-test. n.s., non-significance, **p < 0.01, ***p < 0.001. G Working model. Source data are provided as a Source Data file. G was created with BioRender.com.

Back to article page