Fig. 5: Recombinant SmLAC1 catalyzed the polyermization of catechin and epicatechin in vitro. | Nature Communications

Fig. 5: Recombinant SmLAC1 catalyzed the polyermization of catechin and epicatechin in vitro.

From: Polymerization of proanthocyanidins under the catalysis of miR397a-regulated laccases in Salvia miltiorrhiza and Populus trichocarpa

Fig. 5

a An SDS-PAGE gel image shows the purified recombinant MBP-SmLAC1 protein. b MBP-SmLAC1 protein catalyzed the conversion of catechin (C) and epicatechin (EC) to yellowish compounds. The reactions without SmLAC1 were used as the controls. c UPLC analysis of the products from catechin and epicatechin under the catalysis of SmLAC1. d MS/MS spectra of the products C1, E1, and CE1. e MS/MS spectra of the products C2, E2, and CE2. Source data for Fig. 5a–c are provided in the Source Data file.

Back to article page