Fig. 8: Transcript levels and PA contents in leaves of the control and SmLAC1-overexpressed transgenic S. miltiorrhiza. | Nature Communications

Fig. 8: Transcript levels and PA contents in leaves of the control and SmLAC1-overexpressed transgenic S. miltiorrhiza.

From: Polymerization of proanthocyanidins under the catalysis of miR397a-regulated laccases in Salvia miltiorrhiza and Populus trichocarpa

Fig. 8

a Transcript levels of SmLAC1 in different transgenic lines. Transcripts were determined by qPCR and normalized relative to the expression of SmUBQ10. b Extractable PA levels in leaves of the control and SmLAC1 transgenic lines quantified using DMACA reagent and expressed as epicatechin equivalents. c Non-extractable PA levels evaluated by the butanol-HCl method and expressed as procyanidin B1 equivalents. d–f Elective ion chromatography (EIC) analysis of procyanidins A1, A2, B1, B2, B3, catechin and epicatechin in leaf extracts of the control and SmLAC1-overexpressed transgenic S. miltiorrhiza. g–k. The contents of procyanidins A1, A2, B1, B2, and B3 analyzed using UPLC. l, m Catechin and epicatechin contents in leaves of the control and SmLAC1-overexpressed transgenic S. miltiorrhiza. All data are the average of three independent biological replicates (n = 3). * and ** indicates P < 0.05 and P < 0.01 (One-way ANOVA), respectively. Source data are provided in the Source Data file.

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