Fig. 3: Impact of complete absence of RsbR proteins on the σB response to 3% ethanol.
From: Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis
A Graph showing β-galactosidase activity from PrsbV as a proxy for σB activity (measured at 15-min intervals for 3 h) upon stimulation of the general stress response with 3% ethanol. WT, strain (CSS1113) carrying all four RsbR paralogs. RsbRA only, strain (CSS1480) producing RsbRA as the only RsbR paralog in the cell. No RsbR paralogs, strain (CSS1531) deleted for all four RsbR paralogs. B Schematic showing the yetI and yezB genes, which encode a putative split RsbR paralog, to scale with the rsbRA gene. The deleted region in the ΔyetI-yezB strain is shown with a dashed box. C Graph as in A but also including the trace from a strain (CSS1760) lacking all four rsbR paralogs and deleted for yetI-yezB. In panels A and C, traces are means of biological triplicate experiments, with the shading indicating standard deviation. Source data are provided as a Source Data file.
