Fig. 4: Impact of rsbS deletion alone and with rsbRA deletion on the σB response to 3% ethanol.
From: Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis
A Schematic of the 5’ portion of the rsbRA operon showing the regions deleted in the individual and combined rsbRA and rsbS deletions. B Graph showing β-galactosidase activity from PrsbV as a proxy for σB activity (measured at 15-min intervals for 3 h) upon stimulation of the general stress response with 3% ethanol. WT, strain (CSS1113) carrying all four RsbR paralogs. ΔrsbS, strain (CSS1168) deleted for rsbS as an alternative way to block stressosome formation. C Graph as in B (note different Y-axis scale) but also comparing the ΔrsbRAΔrsbS strain (CSS1392) to a strain harboring the same deletion, but in a background lacking the other RsbR paralogs and RsbP (CSS1441). All traces are means of biological triplicate experiments, with the shading indicating standard deviation. Source data are provided as a Source Data file.
