Fig. 5: Loss of Abhd17b protects against development of liver fibrosis.
a Representative Sirius red staining results of livers from Abhd17b+/+ and Abhd17b−/− mice (n = 3 mice) receiving control olive oil via oral gavage 3 times a week for 4 weeks. Scale bar indicates 50 μm. b Hydroxyproline analysis was performed using mouse liver samples. Error bars represent mean ± SEM (n = 3 mice for olive oil groups, n = 14, 13 mice for CCl4 treatment); 2-tailed unpaired t-test. c Collagen proportionate area (CPA) was calculated based on Sirius red staining. Error bars represent mean ± SEM (n = 3 mice for olive oil groups, n = 14, 12 mice for CCl4 treatment); 2-tailed unpaired t-test. d Representative Sirius red staining results of livers from Abhd17b+/+ (n = 14 mice) and Abhd17b−/− (n = 13 mice) mice recieving CCl4 via oral gavage 3 times a week for 4 weeks. Scale bar indicates 50 μm. e–i Relative mRNA expresion was analyzed for the indicated gene products by qRT-PCR from liver samples. Error bars represent mean ± SEM (n = 3 mice for olive oil groups, n = 14, 13 mice for CCl4 treatment); 2-tailed unpaired t-test. j Representative IHC staining for Pdgfrb, Acta2 and Ck19 in livers from Abhd17b+/+ (n = 2 median mice based on hydroxyproline level) and Abhd17b−/− (n = 2 median mice based on hydroxyproline level) mice recieving either control olive oil or CCl4 via oral gavage 3 times a week for 4 weeks. Scale bar indicates 50 μm. Red arrows indicate examples of Ck19 positive cells. Source data are provided as a Source Data file.
