Fig. 4: Translation efficiency comparison between Frob and Ftri.
a GC3 comparisons of C4 genes across five Flaveria species. As FramPEPC-k1 is missing, its paralog (FramPEPC-k2, FramNA03444, see Supplementary Fig. 4) was utilized as a comparison for Fram in this context. b Abundances of ribosome protected fragment (RPF) of C4 genes. Data are presented as mean values ± SD. (n = 2 biological replicates). c, d Distribution of RPF-to-RNA ratio and protein-to-RPF ratio. e Scatter plots of RPF vs. RNA of Frob and Ftri. Low/high/moderate RPF-to-RNA genes are labeled in pink/light blue/gray. f Scatter plots of protein vs. RPF of Frob and Ftri. Low/high/moderate protein-to-RPF genes were labeled in pink/light blue/gray. Low protein-to-RPF C4 genes were indicated with red arrows. C4 and photorespiratory genes are labeled in red and blue, and Pearson correlation (PC) between RPF vs. RNA, protein vs. RPF is shown in the parentheses on top of each panel in (e) and (f). g, h RPF-to-RNA ratio and protein-RPF ratio for C4 genes and photorespiratory genes. Data are presented as mean values ± SD. Statistical significance was determined by two-tailed Wilcoxon rank sum test (*p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file.
