Fig. 1: Mc3r insufficiency increases systemic and hepatic adiposity in mice.
From: Melanocortin 3 receptor regulates hepatic autophagy and systemic adiposity
a Normalized droplet digital PCR Mc3r transcript levels from Mc3r+/+ liver (n = 37) at ZT12 (6:00 PM) and from Mc3r+/+ liver (n = 35) at ZT2 (8:00 AM) and Mc3rTB/TB liver (n = 18) at ZT12, and from Mc3rTB/TB liver (n = 19) at ZT2. b Normalized Mc3r transcript levels from Mc3r+/+ liver (n = 21) and hypothalamus (n = 4, with each RNA extraction sample combining 6 pooled hypothalamus isolations); c Body weight measured from Mc3r+/+ (n = 8), Mc3rTB/TB (n = 10), MC3RhWT/hWT (n = 12) and MC3RhDM/hDM (n = 11) independent observations. d Total body % fat from Mc3r+/+ (n = 9), Mc3rTB/TB (n = 8), MC3RhWT/hWT (n = 7) and MC3RhDM/hDM (n = 7) independent observations. e Liver triglycerides (TG) from Mc3r+/+ (n = 6), Mc3rTB/TB (n = 5), MC3RhWT/hWT (n = 7) and MC3RhDM/hDM (n = 6) independent observations. f Oil Red O images of livers (n = 2/genotype from two independent observations) g Transmission Electron Microscope (TEM) images of livers from mice in the fed state (n = 1–2/genotype from two independent experiments) at 8–12 weeks of age (h) Quantification of lipid droplet (LD) number from TEM images (n = 16–28 images/genotype from two independent observations) shown in (g). Asterisks indicate LD. Data are represented as mean ± SEM. Groups were compared by one-way ANOVA followed by Tukey’s HSD (a), paired two-tailed Student’s t-test (b, h), two-way ANOVA followed by Tukey’s HSD (c–e). * p < 0.05; ** p < 0.01; *** p < 0.001; male mice (8–12 weeks of age) under chow-fed diet were used unless otherwise indicated. Scale bar, 50 µm (f) and 5 µm (g). Source data are provided as a Source data file.
