Fig. 2: Altered hepatic autophagy and LD recycling in Mc3r insufficient mice.
From: Melanocortin 3 receptor regulates hepatic autophagy and systemic adiposity
a Transmission Electron Microscope images showing liver autophagosomes (AP) from overnight (O/N) fasted Mc3r+/+ and Mc3rTB/TB (n = 1–2/genotype from two independent experiments). b Quantification of AP number from TEM images from both Mc3r+/+ (n = 15–16 images/genotype from two independent observations) shown in (a). c TEM images showing liver AP from overnight (O/N) fasted Mc3rhWT/hWT and MC3RhDM/hDM. d Western blot images of liver lysates from Mc3r+/+, Mc3rTB/TB, Mc3rhWT/hWT and MC3RhDM/hDM. e Quantification of LC3II normalized by actin from both fed-fasted Mc3r+/+ (n = 4) independent observations, Mc3rTB/TB (n = 5) independent observations, MC3RhWT/hWT (n = 10) independent observations, and MC3RhDM/hDM (n = 9 (fed) and 10 (fasted) independent observations) in (d). f Western blot images of primary hepatocytes from Mc3r+/+, Mc3rTB/TB and Mc4r+/− after 1 h serum starvation or 30 min of 10 mM chloroquine (CQ) treatment. g Quantification of AP flux estimated in (f) and Supplementary Fig. 1c from Mc3r+/+ (n = 3), Mc3rTB/TB (n = 3), MC3RhWT/hWT (n = 4) and MC3RhDM/hDM (n = 3). h Fluorescent images of primary hepatocytes from Mc3r+/+, Mc3rTB/TB and MC3RhDM/hDM transgenic mice carrying GFP-LC3 after staining with Lysotracker Red. i Quantification of AP volume in (h) (n = ~1500/2 mice/genotype from two independent experiments). Asterisks indicate AP. Arrows and arrowheads indicate mitochondrial outer and inner membranes, respectively. Data are represented as mean ± SEM. Groups were compared by unpaired two-tailed Student’s t-test (b), ordinary one-way ANOVA followed by Kruskal–Wallis test followed by Dunn’s multiple comparisons test or Tukey’s HSD test (e, g, i). * p < 0.05; ** p < 0.01; *** p < 0.001, Scale bar, 1 µm (a, c) and 10 µm (h). Source data are provided as a Source data file.
